Tag: ELISA kit

Basics About Human MPO/Myeloperoxidase ELISA Kit

Claudia McEwan

Human MPO ELISA kits are a type of laboratory assays that can be used to measure the level of Myeloperoxidase (MPO) in your blood. This article is written for those who have heard about this test and would like more information on how it works.

What is the function of Human MPO?

Myeloperoxidase is produced by granulocytes and monocytes to remove potentially toxic peroxides. It converts hydrogen peroxide (H2O2) to water and oxygen. Hydrogen peroxide is produced by neutrophils and macrophages, and the production of hydrogen peroxide-induced oxidative stress has been implicated in the pathogenesis of inflammatory diseases. To learn more about how this MPO ELISA kit works or to purchase one, please see our website at  www.bosterbio.com/human-mpo-picokine-trade-elisa-kit-ek0850-boster.html.

What causes oxidative stress?. It occurs when cells produce more free radicals than there are neutralizing antioxidants to combat them. These radicals are highly reactive molecules with unpaired electrons causing cell damage or death if they accumulate.

Why is Human MPO important?

Myeloperoxidase (MPO) is a bacterial protein that can be found on the surface of most Gram-negative bacteria. It is an enzyme that breaks down myeloperoxidase, a peroxide compound found on red blood cells. This enzyme can be measured using an ELISA kit to detect MPO levels in the serum of the person being tested for infection.

Differences between human and rat MPO

Monoclonal antibodies are used to detect a protein or enzyme in a test sample. Monoclonal antibodies can only be produced from a single clone of the original cell line. Rats cannot produce monoclonal antibodies, which makes them extremely useful for lab experiments. There are several methods that can be used to detect MPO levels in human samples taken from blood and saliva. Rat MPO is also known as human MPO/Myeloperoxidase ELISA Kit

Conclusion

In conclusion, this article has explained the benefits of using a human MPO/Myeloperoxidase ELISA Kit. For example, the kit can allow for more accurate and precise testing results. It's also possible to use the kit in a qualitative or quantitative manner. 

Know Everything About Mouse BDNF Elisa Kit

Claudia McEwan

Brain-derived neurotrophic factors (BDNF), which is a survival factor induced in cortical neurons, is essential for the survival of striatal cells in the brain. It is a secreted protein that has a molecular weight 27.8kDa and 247 amino acids. It has been shown to be a neuronal survival and differentiation stimulator. BDNF shares a substantial amino acid sequence with nerve growth factor (NGF). 

BDNF were cloned recently and are homologous with NGF. The adult brain contains mRNA products from the NT-3 and BDNF genes, which suggests that these proteins play a role in maintaining the adult nervous system. Long-term potentiation is a critical process that involves BDNF and other neurotrophins. You can buy high –quality mouse BDNF ELISA equipment through the internet.

LTP induced by BDNF is affected postsynaptically. BDNF has a trophic effect on the central nervous system's serotonergic (5HT) neurons. BDNF plays an important maintenance role in anxiety-related behavior regulation and food intake via central mediators in the fasted and basal states. It is used to treat breathing disorders like spinal injury-related respiratory insufficiency. 

Mouse BDNF ELISA Kit uses standard sandwich enzyme-linked immuno-sorbent technology. Precoated 8 x 12-divisible strips with mouse BDNF-specific polyclonal antibodies are available. Biotinylated monoclonal mouse detection antibodies are available. After preparing the test samples, biotinylated detection antibodies are added to the wells.

The wells then need to be washed with a PBS/TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. To visualize the HRP enzyme reaction, TMB (HRP substrate) is used. TMB is catalyzed in HRP to create a blue color product which changes to yellow after adding acidic solution.