Brain-derived neurotrophic factors (BDNF), which is a survival factor induced in cortical neurons, is essential for the survival of striatal cells in the brain. It is a secreted protein that has a molecular weight 27.8kDa and 247 amino acids. It has been shown to be a neuronal survival and differentiation stimulator. BDNF shares a substantial amino acid sequence with nerve growth factor (NGF).
BDNF were cloned recently and are homologous with NGF. The adult brain contains mRNA products from the NT-3 and BDNF genes, which suggests that these proteins play a role in maintaining the adult nervous system. Long-term potentiation is a critical process that involves BDNF and other neurotrophins. You can buy high –quality mouse BDNF ELISA equipment through the internet.
LTP induced by BDNF is affected postsynaptically. BDNF has a trophic effect on the central nervous system's serotonergic (5HT) neurons. BDNF plays an important maintenance role in anxiety-related behavior regulation and food intake via central mediators in the fasted and basal states. It is used to treat breathing disorders like spinal injury-related respiratory insufficiency.
Mouse BDNF ELISA Kit uses standard sandwich enzyme-linked immuno-sorbent technology. Precoated 8 x 12-divisible strips with mouse BDNF-specific polyclonal antibodies are available. Biotinylated monoclonal mouse detection antibodies are available. After preparing the test samples, biotinylated detection antibodies are added to the wells.
The wells then need to be washed with a PBS/TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. To visualize the HRP enzyme reaction, TMB (HRP substrate) is used. TMB is catalyzed in HRP to create a blue color product which changes to yellow after adding acidic solution.